
Contents
49.10.02
AOAC Official Method 991.26
Domoic Acid in Mussels: Liquid Chromatographic Method
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Published:January 2023
Cite
Domoic Acid in Mussels: Liquid Chromatographic Method', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1991
Final Action 1999
(Applicable to determination at ca 20 μg domoic acid/g tissue.)
Results of the interlaboratory study supporting acceptance of the method:
sr = 3.5–3.6; sR = 1.8–23.9; RSDr = 1.9–4.8%; RSDR = 7.5–19.4%
Principle
Domoic acid is extracted from homogenized mussel tissue by boiling for 5 min with 0.1 M HCl. Mixture is cooled and centrifuged, and an aliquot of supernate is diluted, filtered, and analyzed by isocratic LC with UV detection at 242 nm with mobile phase of CH3CN–H2O acidified to pH ca 2.5.
Apparatus
Equivalent apparatus may be substituted.
...
Reagents
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Preparation of Test Solution
Extract and analyze test portion on same day. Refrigerate test samples and extracts when not being handled. (Note: Domoic acid in acidic extracts slowly decomposes if left at room temperature.)
Prepare homogenate as in 959.08D(a) (see 35.1.37). Accurately weigh ca 50 g homogenate in clean 250 mL beaker. Add 50 mL 0.1 M HCl and stir thoroughly. Quickly heat mixture to boiling (within 10 min) and continue gentle boiling, with stirring, for exactly 5 min. Immediately transfer hot beaker and contents to ice bath and let cool to room temperature (ca 10 min).
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