
Contents
9.2.23
AOAC Official Method 977.15
Mercury in Fish Alternative Flameless Atomic Absorption: Spectrophotometric Method
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Published:January 2023
Cite
Mercury in Fish Alternative Flameless Atomic Absorption: Spectrophotometric Method', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1977
Final Action 1978
[Rinse all glassware before use with HNO3 (1 + 9).]
Apparatus
See 971.21A(a) , (b), and (e) (see 9.2.22), and in addition:
...
Determination
Weigh 5.0 g (wet weight) test portion thoroughly mixed fish material into digestion flask, 971.21A(e) (see 9.2.22). Rinse neck of flask with <5 mL H2O, if necessary. Add ca 20 boiling stones, A(a), 10–20 mg V2O5, and 20 mL H2SO4–HNO3 (1 + 1). Quickly connect flask to condenser, A(d), and swirl to mix. Circulate cold H2O through condenser during digestion. Apply sufficient heat (luminous flame is suitable) to produce low initial boil (ca 6 min) and finish digest ion with strong boil (ca 10 min). Swirl flask intermittently during digestion. No solid material should be apparent except for globules of fat after ca 4 min.
Remove flask from heat and wash condenser with 15 mL H2O. Add two drops 30% H2O2 through condenser and wash into flask with 15 mL H2O. Cool digested fish solution to room temperature by placing flask, still connected to condenser, in beaker of H2O. Disconnect flask, rinse ground joint with H2O, and quantitatively transfer digest to 100 mL volumetric flask. Ignore solidified fat; it does not interfere. Carefully rinse digestion flask with several portions H2O and dilute to volume with rinse H2O.
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