
Contents
16.17.01
AOAC Official Method 984.29
Howard Mold CountingGeneral Instructions
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Published:January 2023
Cite
Howard Mold CountingGeneral Instructions', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1984
See Figure 984.29A .
Diagnostic Characteristics of Mold
Before attempting to make mold count, analysts should be familiar with cellular structure of product. They can do this through direct microscopic examination of healthy tissue excised from raw product or through study of reference books. Analysts should assume that any product can be contaminated with a variety of vegetable, animal, and synthetic fibers. Presence of such materials greatly increases probability for misidentification of molds.
It is essential that analyst be able to distinguish hyphae and look-alikes with which they might be confused. Although many filaments can be easily and accurately recognized as mold, others require more careful appraisal before they can be reported as mold. Only rarely are all mold characteristics given here present at one time; usually 2 or 3 are absent. Features mentioned below are observed at 100–450×.
...
Determination
Clean Howard cell, 945.75B(m )(1) (see 16.1.01), so that Newton’s rings are produced between slide and cover glass. Remove cover and with knife blade or scalpel, place portion of well-mixed test sample on central disk; with same instrument, spread evenly over disk, and cover with glass so as to give uniform distribution. Use only enough test portion to bring material to edge of disk. (It is of utmost importance that portion be taken from thoroughly mixed test sample and spread evenly over slide disk. Otherwise, when cover slip is put in place, insoluble material, and consequently molds, may be more abundant at center of mount.) Discard any mount showing uneven distribution or absence of Newton’s rings, or liquid that has been drawn across moat and between cover glass and shoulder.
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