
Contents
45.1.22
AOAC Official Method 982.29
Vitamin D in Mixed Feeds, Premixes, and Pet Foods: Liquid Chromatographic Method
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Published:January 2023
Cite
Vitamin D in Mixed Feeds, Premixes, and Pet Foods: Liquid Chromatographic Method', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1982
Final Action 1983
[Applicable to all food products containing >2 IU and <200 IU vitamin D/g. For products containing ≥200 IU vitamin D/g, use 980.26 (see 45.1.19).]
Principle
Products are saponified and extracted, and unsaponifiable material is chromatographed successively on alumina to remove tocopherols and carotenes, if present, and on LC cleanup column to separate from interfering substances. Second LC column packed with silica separates vitamin D from impurities. Vitamin D is corrected for amount previtamin D formed during saponification. Vitamin D is sum of vitamin D and previtamin D.
Reagents
...
Alumina Column
Seal coarse fritted glass disk in lower end of 150 × 20 mm (id) tube and 250 mL bulb at upper end. Fit constricted portion at lower end with Teflon stopcock.
Heat 250 g alumina overnight at 750°C. Cool and store in vacuum desiccator. Weigh 30 g dried alumina into 100 mL Erlenmeyer. Pipet 2.7 mL H2O into flask, and stopper. Heat 5 min on steam bath. Vigorously shake warm flask until powder is free-flowing. Cool and let stand 30 min.
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