
Contents
44.4.18A
AOAC Official Method 998.12
C-4 Plant Sugars in Honey: Internal Standard Stable Carbon Isotope Ratio Method
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Published:January 2023
Cite
C-4 Plant Sugars in Honey: Internal Standard Stable Carbon Isotope Ratio Method', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1998
Revised First Action 2013
See Table 998.12 for results of the interlaboratory study supporting acceptance of the method.
Statistical analysis of interlaboratory results for honey stable carbon isotope ratio
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Principle
Addition of C-4 sugars (cane sugar/corn syrup) to honey increases the value of the stable carbon isotope ratio of the honey. Stable carbon isotope ratio value for protein isolated from honey provides a standard to which stable carbon isotope ratio value of the whole honey is compared. The difference between these values (the ISCIRA index) allows for the detection and an estimate of the amount of the C-4 sugar content of honey.
Both honey and protein must be analyzed on the same instrument.
Honey Alternative I—Batchwise Method
Final Action 1979
Apparatus
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Preparation of Honey
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While tubes are still warm, place 1–6 analyte tubes on vacuum manifold and evacuate by mechanical pump 3–4 min, then seal tubes with torch. Place tubes horizontally in oven with loose CuO covering test portion and bottom of each tube end-to-end. Combust test portions at 585–590°C for 1 h. Let tubes cool in oven at least 1 h at <400°C. Attach tubes to vacuum purification line, and break seal or open with a tube cracker [see Anal. Chem. 48, 1652(1976)].
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