
Contents
7.3.10
AOAC Official Method 977.07
Fluometuron in Pesticide Formulations: Gas Chromatographic Method
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Published:January 2023
Cite
Fluometuron in Pesticide Formulations: Gas Chromatographic Method', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1977
Final Action 1978
Standard Solutions
...
Preparation of Test Solution
Accurately weigh test portion containing ca 125 mg fluometuron into 2 oz (50 mL) round bottle with Teflon-lined or Poly-Seal screw cap. Pipet in 25 mL diethyl phthalate internal standard solution and shake well. Pipet in 3 mL trifluoroacetic anhydride and shake mechanically 15 min; then place bottle in 55°C water bath 30 min. Let cool to room temperature. Let insoluble materials settle or centrifuge portion of extract to obtain clear solution.
Gas Chromatography
Use instrument equipped with flame ionization detector and 1.83 m × 2 mm (id) glass column packed with 2% OV-3 on 80–100 mesh Gas-Chrom Q. Condition 24 h at 240°C with N2 or He at ca 40 mL/min. Column should have ≥1500 theoretical plates. Use on-column injection to prevent decomposition of derivative.
Typical operating conditions: temperatures—inlet 150°C, column 115 ± 10°C, detector 250°C; N2 or He carrier gas, 20–22 mL/min; air and H2 as specified by manufacturer; attenuation varied so that peak heights of pesticide and internal standard are 60–80% full scale. Retention times for fluometuron derivative and diethyl phthalate are 3–5 and 8–10 min, respectively.
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