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Book cover for Official Methods of Analysis of AOAC INTERNATIONAL (22nd Edition) Official Methods of Analysis of AOAC INTERNATIONAL (22nd Edition)

Contents

SMPRs®  AOAC SMPR®   2017.021 Quantitation of Wheat, Rye, and Barley Gluten in Oats

Revised March 2019 to provide better final calculations (wet chemical analysis vs Dumas nitrogen content method) used to determine gluten content for wheat, rye, and barley used for spiking samples

AOAC SMPR®   2018.012 Quantitation of Peanut by ELISA-Based Methods

Revised March 2019 to correct “1 ppm peanut protein” to “2 ppm peanut protein” in Annex

NEW! AOAC SMPR®   2019.001 Detection of Aspergillus in Cannabis and Cannabis Products

NEW! AOAC SMPR®   2019.002 Identification and Quantitation of Selected Residual Solvents in Dried Cannabis Materials

NEW! AOAC SMPR®   2019.003 Quantitation of Cannabinoids in Plant Materials of Hemp (Low THC Varieties Cannabis spp.)

NEW! AOAC SMPR®   2019.004 Quantitation of Furan and Alkyl Furans in Selected Matrices

NEW! AOAC SMPR®   2020.001 Determination of Heavy Metals in a Variety of Cannabis and Cannabis-Derived Products

NEW! AOAC SMPR®   2020.002 Detection of Salmonella species in Cannabis and Cannabis Products

NEW! AOAC SMPR®   2020.003 Determination of 2’-Fucosyllactose (2’FL) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2020.004 Determination of Lacto-N-Neotetraose (LNnT) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2020.005 Determination of Bovine Lactoferrin in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2020.006 Nontargeted Testing of Ingredients for Food Authenticity/Fraud Evaluation of Honey

NEW! AOAC SMPR®   2020.007 Nontargeted Testing of Ingredients for Food Authenticity/Fraud Evaluation of Extra Virgin Olive Oil

NEW! AOAC SMPR®   2020.008 Nontargeted Testing of Ingredients for Food Authenticity/Fraud Evaluation of Pasteurized Whole Liquid Bovine Milk

NEW! AOAC SMPR®   2020.009 Targeted Testing of Barley and Malt Extract, Beet Sugar Syrup, Corn and Cane Sugar Syrup, C-4 Plant Sugar, and High-Fructose Corn Sugar for Adulteration of Floral and Acacia Honey

NEW! AOAC SMPR®   2020.010 Targeted Testing of Other Vegetable Oils and Low-Quality Olive Oils as Adulterants for Evaluation of Extra Virgin Olive Oil (EVOO)

NEW! AOAC SMPR®   2020.011 Targeted Testing of Formaline/Formaldahyde, Starch, and Soy Protein as Adulterants for Evaluation of Liquid Raw Bovine Milk

NEW! AOAC SMPR®   2020.012 Detection of Shiga Toxin-Producing Escherichia coli in Cannabis and Cannabis Products

NEW! AOAC SMPR®   2020.013 Mycotoxin Screening Technique in Cannabis Plant Material and Cannabis Derivatives

NEW! AOAC SMPR®   2020.014 Characterization and Quantitation of Neutral Components from Glycerol Esters of Wood Rosin (GEWR)

NEW! AOAC SMPR®   2020.015 Characterization and Quantitation of Monoesters from Glycerol Esters of Wood Rosin (GEWR)

NEW! AOAC SMPR®   2020.016 Characterization and Quantitation of Component Fractions from Glycerol Esters of Wood Rosin (GEWR)

NEW! AOAC SMPR®   2020.017 Characterization and Quantitation of Residual Resin Acids from Glycerol Esters of Wood Rosin (GEWR)

NEW! AOAC SMPR®   2021.001 Determination of Chlorate and Perchlorate in Baby Foods, Infant/Adult Formulas, and Their Ingredients

NEW! AOAC SMPR®   2021.002 Determination of Vitamin C (L-Ascorbic Acid) in All Forms of Infant Formula, Follow-Up Formula, Baby Foods, Adult/Pediatric Formula, Complementary Food Supplements, Nutritional Supplements for Pregnant Women and Nursing Mothers, and Sports Nutrition Food

NEW! AOAC SMPR®   2021.003 Determination of 3’-Sialyllactose (3’-SL) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2021.004 Determination of 3-Fucosyllactose (3-FL) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2021.005 Determination of 6’-Sialyllactose (6’-SL) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2021.006 Determination of Difucosyllactose (DFL) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2021.007 Determination of Lacto-N-Tetraose (LNT) in Infant and Adult/Pediatric Nutritional Formula

NEW! AOAC SMPR®   2021.008 Glyphosate, Its Metabolites, and Trimesium in Fruits and Vegetables, Cereals, Food of Animal Origin, Pet food and Baby Food

NEW! AOAC SMPR®   2021.009 Viable Yeast and Mold Count Enumeration in Cannabis and Cannabis Products

Chapter 4       NEW!   2018.07 (4.7.08) Fructan (Inulin, FOS, Levan, and Branched Fructan) in Animal Food (Animal Feed, Pet Food, and Ingredients)

Chapter 9        2016.04 (9.2.43) Arsenic Speciation in Fruit Juice Final Action 2019

Chapter 17        2015.13 (17.2.12) Enumeration of Aerobic Bacteria in Food

D(11): Deleted “Average the counts between the replicate plates” for clarity

NEW!   2018.02 (17.2.13) Enumeration of Yeast and Mold in Select Foods

NEW!   2019.02 (17.2.14) Enumeration of Total Aerobic Count in Select Foods

NEW!   2018.05 (17.3.11) Enumeration of Enterobacteriaceae in Select Foods and Environmental Surfaces

Final Action 2021

NEW!   2018.13 (17.4.11) Enumeration of Escherichia coli and Coliform in a Broad Range of Foods and Select Environmental Surfaces

Final Action 2021

NEW!   2019.03 (17.4.12) Escherichia coli O157:H7 in Select Foods

NEW!   2020.06 (17.4.13) Enterohemorrhagic E. coli in Select Foods

993.06 (17.5.04) Staphylococcal Enterotoxins in Selected Foods

Repealed 2019: Method uses 3M TECRA products that have been discontinued

989.14 (17.9.13) Salmonella in Foods

Repealed 2019: Method uses 3M TECRA products that have been discontinued

998.09 (17.9.24) Salmonella in Foods

Repealed 2019: Method uses 3M TECRA products that have been discontinued

2016.01 (17.9.41) Salmonella spp. in Select Foods and Environmental Surfaces

Final Action 2019

2017.09 (17.9.42) Confirmation and Identification of Salmonella species, Cronobacter species,

Campylobacter species, and Other Gram-Negative Organisms

Final Action 2020

Minor modification 2020

Included Revised First Action J. AOAC Int. reference

  2018.01 (17.9.44) Cronobacter species in Select Foods and Environmental Surfaces

Final Action 2021

Included J. AOAC Int. reference

NEW!   2019.01 (17.9.45) Cronobacter species in Select Foods and Environmental Samples

NEW!   2020.02 (17.9.46) Salmonella species in a Broad Range of Foods and Select Environmental Samples

NEW!   2020.03 (17.9.47) Salmonella species in Select Foods

NEW!   2021.02 (17.9.48) Detection of Salmonella species in Broad Range of Foods and Selected

Environmental Surfaces

995.22 (17.10.06) Listeria in Foods

Repealed 2019: Method uses 3M TECRA products that have been discontinued

2002.09 (17.10.06A) Listeria in Foods

Repealed 2019: Method uses 3M TECRA products that have been discontinued

2016.07 (17.10.18) Listeria species in Select Foods and Environmental Surfaces

Final Action 2019

2016.08 (17.10.19) Listeria monocytogenes in a Variety of Foods and Select Environmental Surfaces

Final Action 2019

2017.10 (17.10.20) Confirmation and Identification of Listeria monocytogenes, Listeria species, and Other Gram-Positive Organisms

Final Action 2020

Minor modification 2020

NEW!   2019.10 (17.10.21) Listeria species in a Variety of Foods and Select Environmental Samples

NEW!   2019.11 (17.10.22) Listeria monocytogenes in a Variety of Foods and Select Environmental Samples

Chapter 22       NEW!   2018.10 (22.1.12) Cannabinoids in Cannabis sativa Dried Flowers and Oils

NEW!   2018.11 (22.1.13) Quantitation of Cannabinoids in Cannabis Dried Plant Materials, Concentrates, and Oils

Revised First Action 2020: Modified for extension to hemp and to include procedure for sample dry-weight determination

Chapter 23        2013.07 (23.1.21) Determination and Identification of Nicarbazin in Chicken Tissues

Final Action 2021: Method applicability changed from “confirmation” to “identification” and representative chromatogram was added, among other changes

NEW!   2020.04 (23.1.22) Screening of 154 Veterinary Drug Residues in Animal Source Foods

Chapter 29        2017.15 (29.1.30) Bisphenol A (BPA) in Commercially Packaged Ready-to-Consume Carbonated and Noncarbonated Water and Nonalcoholic Beverages

Included J. AOAC Int. reference

NEW!   2019.07 (29.1.31) Free Bisphenol A (BPA) in Commercially Packaged, Ready-to-Consume,

Carbonated/Noncarbonated Water and Nonalcoholic Beverages

Chapter 30       NEW!   2019.04 (30.1.38) Ethanol in Kombucha Tea Products

NEW!   2019.08 (30.1.39) Ethanol Concentration in Kombucha Beverages

Chapter 31       NEW!   2020.05 (31.5.17) Flavanol and Procyanidin (by Degree of Polymerization 1–7) of Cocoa-Based Products

Chapter 32        2012.01 (32.1.44) Gliadin as a Measure of Gluten in Food

Modification to include matrix extension

2015.05 (32.2.11) Partially Hydrolyzed Gluten in Fermented Cereal-Based Products

Final Action 2018: Revised applicability statement to include “and may not measure or detect all fermented and/or hydrolyzed forms of gluten”

NEW!   2017.17 (32.2.13) Detection and Quantitation of Selected Food Allergens

NEW!   2018.15 (32.2.14) Gluten from Wheat, Rye, and Barley in Oats and Oat Products

Chapter 33       NEW!   2020.01 (33.2.69) Lactose in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients

NEW!   2020.08 (33.2.70) Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients

Final Action 2021

NEW!   2020.09 (33.2.71) Lactose in Free or Low-Lactose Dairy Products

Chapter 35        2014.08 (35.1.46) Polycyclic Aromatic Hydrocarbons (PAHs) in Seafood

Final Action 2018

Chapter 44       NEW!   2018.16 (44.1.31) Sugar Profile in Food, Dietary Supplements, Pet Food, and Animal Feeds

Chapter 45        2017.16 (45.4.18) Total Dietary Fiber in Foods Final Action 2020

Included J. AOAC Int. reference

Chapter 50        2011.06 (50.1.29) Total Folate in Infant Formula and Adult Nutritionals

Final Action 2018

Codex Type II 2019

Included J. AOAC Int. Final Action reference

2011.14 (50.1.37) Minerals and Trace Elements in Milk and Milk Products, Infant Formula, and Adult Nutritionals

Final Action 2018 (for infant formula and adult nutritionals only; matrix extension to infant formula)

2015.09 (50.2.08) trans and Total (cis+trans) Vitamin K1 in Infant, Pediatric, and Adult Nutritionals

Final Action 2018

Codex Type II, AOAC-ISO Method 2019

Included Final Action J. AOAC Int. reference

2015.14 (50.2.09) Simultaneous Determination of Total Vitamins B1, B2, B3, and B6 in Infant Formula and Related Nutritionals

Final Action 2019

AOAC-ISO Method

Included Revised First Action J. AOAC Int. reference

2015.10 (50.6.05) Free and Total Choline and Carnitine in Infant Formula and Adult/Pediatric Nutritional Formula

Final Action 2019

AOAC-ISO Method

2015.06 (50.10.01) Minerals and Trace Elements in Milk, Milk Products, Infant Formula, and Adult/Pediatric Nutritional Formula

Codex Type II, AOAC-ISO/IDF Method 2019

2016.03 (50.11.03) Chloride in Milk, Milk Powder, Whey Powder, Infant Formula, and Adult Nutritionals

Codex Type II, AOAC-ISO/IDF Method

Included Final Action J. AOAC Int. reference

2016.14 (50.13.02) Fructans in Infant Formula and Adult Nutritionals

Final Action 2020

AOAC-ISO/IDF Method

2016.13 (50.14.01) Lutein, β-Carotene, and Lycopene in Infant Formula and Adult Nutritionals

Final Action 2019 (for β-carotene and lycopene only)

AOAC-ISO Method

2017.03 (50.15.01) Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula

Final Action 2019

NEW!   2018.06 (50.15.02) Total Amino Acids in Infant Formulas and Adult Nutritionals

NEW!   2019.09 (50.15.03) Total Proteinogenic Amino Acids and Taurine in Infant Formula and Adult/Pediatric Formula

NEW!   2018.03 (50.16.01) 2- and 3-MCPD, 2- and 3-MCPD Esters, and Glycidyl Esters (GE) in Infant and Adult/Pediatric Nutritional Formula

NEW!   2018.12 (50.16.02) 2-Monochloropropanediol (2-MCPD), 3-Monochloropropanediol (3-MCPD), and Glycidol in Infant and Adult/Pediatric Nutritional Formula

NEW!   2021.01 (50.17.01) β-Galactooligosaccharides in Infant Formula and Adult Nutritionals

Chapter 51       NEW!   2018.14 (51.13.02) Quantitation of Aloe Vera Polysaccharides NEW!   2018.04 (51.21.01) Select Nonvolatile Ginger Constituents in Dietary Ingredients and Finished Dosage Forms

NEW!   2019.05 (51.22.01) A-Type Proanthocyanidins in Cranberry‐Based Foods and Dietary Supplements

NEW!   2019.06 (51.22.02) Soluble Proanthocyanidins in Cranberry Foods and Dietary Supplements

Appendix P NEW!  Guidance for Soil Collection, Characterization, and Application for Biothreat Agent Detection Methods and Site Evaluations

Appendix Q NEW!  Recommendations for Developing Molecular Assays for Microbial Pathogen Detection Using Modern In Silico Approaches

Appendix R NEW!  Guidelines for Verifying and Documenting the Relationships Between Microbial Cultures

If you have any questions of a technical nature or suggestions for editorial changes, please e-mail us at [email protected].  

May 2023

AOAC SMPR® 2022.001 Quantitation of Cannabinoids in Beverages

AOAC SMPR® 2022.002 Determination of Folate in Dietary Supplements

AOAC SMPR® 2022.003 Determination of Biological Spices and Botanicals and Relevant (Common) Biological Adulterants

AOAC SMPR® 2022.004 Determination of Fatty Acids Which Are Esterified at SN-2 Position (Beta) of Triacylglycerol Molecules in Infant and Adult/Pediatric Nutritional Formula

AOAC SMPR® 2022.005 A1- and A2-Type Beta-Casein in Infant Formulas and Adult Nutritionals

AOAC SMPR® 2022.006 Acrylamide Acrylamide in Potato Products, Baby Food, Bread, Other Cereal and Bakery Products, Cocoa Products, Coffee, Tea, Herbs and Spices (Including Their Mixtures), Dry Pet Food, and Nuts

Appendix L “AOAC Recommended Guidelines for Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Single-Laboratory Validation”

June 2023

997.02 Yeast and Mold Counts in Foods and Dried Cannabis Flower (Revised First Action for Cannabis Flower, THC >0.3%, Only)

2009.03  Salmonella in Foods and Environmental Surfaces (Revised 2021: Minor modification to Rotor-Gene Q software)

2014.05 Enumeration of Yeast and Mold in Foods, Selected Surfaces, and Dried Cannabis Flower (Revised First Action for Cannabis Flower, THC > 0.3%, and Selected Surfaces Only)

2015.13 Enumeration of Aerobic Bacteria in Food and on Selected Surfaces (Revised First Action for Selected Surfaces Only)

2017.07 Ethanol in Food by Enzytec™ Liquid Ethanol (Final Action)

2018.06 Total Amino Acids in Infant Formula and Adult Nutritionals (Final Action)

2019.01  Cronobacter species in Select Foods and Environmental Samples (Final Action and Minor Modification)

2019.03  E. coli O157:H7 in Select Foods (Final Action and Minor Modification)

2019.10  Listeria Species in a Variety of Foods and Select Environmental Samples (Final Action and Minor Modification)

2019.11  Listeria monocytogenes in a Variety of Foods and Select Environmental Samples (Final Action and Minor Modification)

2020.02  Salmonella Species in a Broad Range of Foods and Select Environmental Samples (Minor Modification)

2020.05 Cocoa Flavanols and Procyanidins [by Degree of Polymerization (DP) 1–7] in Cocoa-Based Products (Final Action)

2020.06 Enterohemorrhagic E. coli in Select Foods (Minor modification)

2020.07 Available Carbohydrates in Cereal and Cereal Products, Dairy Products, Vegetables, Fruit, and Food Products and Animal Feeds (Final Action)

2020.08 Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients (Final Action)

2020.09 Lactose in Lactose-Free or Low-Lactose Dairy Products (Final Action)

2021.03 Heavy Metals in a Variety of Cannabis and Cannabis-Derived Products (First Action)

2021.04 Measurement of Water Activity in the Presence of High Volatile Concentrations (First Action)

2021.05  Listeria monocytogenes in a Broad Range of Foods and Selected Environmental Surfaces (First Action)

2021.06  Listeria species in a Broad Range of Foods and Selected Environmental Surfaces (First Action)

2021.07 Analysis of Bovine Lactoferrin in Infant Formula and Adult Nutritional Products (First Action)

2021.08  Cronobacter species in Infant Formulas, Infant Cereals, Ingredients, and Environmental Samples (First Action)

2022.01 Insoluble, Soluble, and Total Dietary Fiber in Foods and Food Ingredients (First Action)

2022.02 Analysis of Six Human Milk Oligosaccharides (HMOs) in Infant Formula and Adult Nutritionals (First Action)

NOVEMBER 2023

AOAC SMPR®  2023.001 Pesticides in Cannabis-Containing Beverages

AOAC SMPR® 2023.002 Pyrrolizidine Alkaloids in Teas, Herbal Infusions, Dried Herbs, Seed Spices, Honey, and Botanical Dietary Supplements and Ingredients

AOAC SMPR® 2023.003 Per- and Polyfluoroalkyl Substances (PFAS) in Produce, Beverages, Dairy Products, Eggs, Seafood, Meat Products, and Feed

2005.04  Escherichia coli O157:H7 in Selected Foods

2009.03  Salmonella in Foods and Environmental Surfaces

2018.16 Sugar Profile in Food, Dietary Supplements, Pet Food, and Animal Feeds (Final Action 2023)

2022.03 Taurine in Infant Formulas and Adult Nutritionals (First Action)

2022.04 2′-Fucosyllactose (2′-FL), 3-Fucosyllactose (3-FL), 6′-Sialyllactose(6′-SL), 3′-Sialyllactose (3′-SL), Lacto-N-Tetraose (LNT), and Lacto-N-neoTetraose (LNnT) in Infant Formula and Adult Nutritionals (First Action)

Appendix G: Procedures and Guidelines for the Use of AOAC Voluntary Consensus Standards to Evaluate Characteristics of a Method of Analysis

Appendix T: Standard Requirements for Nucleotide Sequences Used in Biothreat Agent Detection, Identification, and Quantification: Verified Next-Generation Sequences (VNGS)

January 2024

AOAC SMPR ® 2023.004 Selected Residual Solvents in Color Additives from Crop-Based Sources

AOAC SMPR ® 2023.005 Heavy Metals in Cannabis-Containing Beverages

AOAC SMPR® 2023.006 Vanillin, Ethyl Vanillin, Methyl Vanillin, and Coumarin in Infant Formulas and Their Ingredients

2017.01  Escherichia coli O157:H7 in Selected Foods

March 2024

AOAC SMPR® 2024.001 Selected Pesticides in Color Additives from Crop-Based Sources

2018.16 Sugar Profile in Food, Dietary Supplements, Pet Food, and Animal Feeds (Final Action 2023)

2018.17 Kavalactones and Flavokavains in Kava (Piper methysticum Forst. F.) Dietary Supplements and Dietary Ingredients (First Action 2018)

2020.01 Lactose in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients (Final Action 2023)

2021.09 Total Sulfites in Shrimp (First Action 2021)

2022.05 Fluoride in Milk, Soy, and Water-Based Infant and Adult Nutritional Formulas (First Action 2022)

2022.06 Chlorate and Perchlorate in Baby Foods, Infant and Adult Formulas, and Their Ingredients (First Action 2022)

2022.07 Seven Human Milk Oligosaccharides in Infant Formula and Adult Nutritionals (First Action 2022)

Appendix U: Laboratory Guidance: Drying Field-Fresh Hemp Plant Samples in Preparation for Determination of Total THC on a Dry-Weight Basis”

May 2, 2024

AOAC SMPR® 2024.002 Determination of Trace Elemental Contaminants in Food and Beverages

2023.01 Acrylamide in Coffee, Cereals, Baby Food, Cocoa, Dry Pet Food, Potato Products, Vegetable Crisps, Biscuit, Tea, Nuts, and Spices (First Action 2023)

Appendix M: Guidance on Food Allergen Immunoassay Validation

July 2024

2016.16 Curcuminoids in Turmeric Raw Materials and Dietary Supplements (Final Action 2023)

2020.04 Screening of 152 Veterinary Drug Residues in Foods of Animal Origin (Final Action 2023)

2021.01 β-Galactooligosaccharides (GOS) in Infant Formula (Final Action 2023)

2021.10 Bovine Lactoferrin in Infant Formula and Pediatric/Adult Nutritionals (First Action 2021)

October 2024

AOAC SMPR® 2024.003 Detection, Identification, and Characterization of Cyclospora cayetanensis

AOAC SMPR® 2024.004 Detection of Multiple Biothreat Agent Organisms in Environmental Samples by Amplicon Sequencing

AOAC SMPR® 2024.005 Determination of Ethylene Oxide and Its Marker Residue 2-Chloroethanol in Oil Seeds and Nuts, Grains, Pulses, Dried Herbs, Spices and Extracts Thereof, Food Additives, Dietary Supplement Products and Ingredients, Dried Fruits, Essential Oils, Coffee, Cocoa and Composite Foods

2021.07 Analysis of Bovine Lactoferrin in Infant Formula and Adult Nutritional Products (Final Action 2024)

2022.01 Insoluble, Soluble, and Total Dietary Fiber in Foods and Food Ingredients (Final Action 2024)

November 2024

999.06  Listeria in Foods and Environmental Surfaces (Revised 2024)

2004.06  Listeria in Foods and Environmental Surfaces (Revised 2024)

2011.03  Salmonella in a Variety of Food and Environmental Surfaces (Revised 2024)

2013.01  Salmonella in a Variety of Foods (Revised 2024)

2021.02  Salmonella Species in a Broad Range of Foods and Selected Environmental Surfaces (Final Action 2023 for Manual Workflow; First Action 2024 for Automated Workflow)

2021.05  Listeria monocytogenes in a Broad Range of Foods and Selected Environmental Surfaces (Final Action 2023 for Manual Workflow; First Action 2024 for Automated Workflow)

2021.06  Listeria species in a Broad Range of Foods and Selected Environmental Surfaces (Final Action 2023 for Manual Workflow; First Action 2024 for Automated Workflow)

2021.09 Total Sulfites in Shrimp (Final Action 2023)

2021.10 Bovine Lactoferrin in Infant Formula and Pediatric/Adult Nutritionals (Final Action 2024)

2022.08 Taurine in Infant Formulas and Adult/Pediatric Nutritionals (First Action 2022)

January 2025

2011.17  Salmonella, Escherichia coli, and Other Enterobacteriaceae (Revised December 2024/Minor Modification: To include VITEK COMPACT PRO instrument)

2012.02 Gram-Positive Bacteria Identification (Revised December 2024/Minor Modification: To include VITEK COMPACT PRO instrument)

2016.05 Vitamin D2 and Vitamin D3 in Fortified Milk Powders, Infant Formulas, and Adult/Pediatric Nutritional Formulas (Revised June 2024/Minor Modification: To change “70°C” to “80°C” in B(g) and G(c) to ensure that vitamin D from internal standard and sample have both reached equilibrium.)

2017.03 Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula (Revised June 2024/Minor Modification: To use 1/x weighting for calibration in F(c) and F(d)(3); update part numbers for syringe filters in B(e), sodium phosphate monobasic in C(b), and phosphoric acid in C(c); and add Caution statements.)

2024.09 Folic Acid in Various Dietary Supplement Dosage Forms (First Action 2024)

February 2025

AOAC SMPR® 2024.006 Determination of Milk Fat Globule Membrane Proteins in Infant and Adult/Pediatric Nutritional Formula

March 2025

Appendix L: AOAC Recommended Guidelines for Stakeholder Program on Infant Formula and Adult Nutritionals (SPIFAN) Single-Laboratory Validation

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