
Contents
17.10.02
AOAC Official Method 992.18
Listeria species: Biochemical Identification Method
(MICRO-ID Listeria)
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Published:January 2023
Cite
Listeria species: Biochemical Identification Method
(MICRO-ID Listeria)', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1992
Final Action 1996
(For presumptive generic identification of Listeria species isolated from foods and environmental test samples. Use of commercial biochemical kit as alternative to conventional biochemical testing is based on demonstration in analyst’s laboratory of adequate correlation between biochemical kit and conventional biochemical tests as described in current edition of Bacteriological Analytical Manual.)
See Table 992.18A for results of the interlaboratory study supporting acceptance of the method.
Principle
Method depends on principle that test inoculum contains preformed enzymes which can be detected after 24 h at 37°C. Test strip contains filter paper discs impregnated with chemical substrate/reagents specific for enzymes endogenous to Listeria spp. Substrates are converted to metabolic end products which react with detection system to produce visible color changes. Tests included in strip are listed in Table 992.18B. Each test is reported as either positive or negative. Typical results for each Listeria spp. are given in Table 992.18C.
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