
Contents
17.9.39
AOAC Official Method 2013.14
Identification of Salmonella spp. from Colony Picks: ANSR® Salmonella Confirmation Test
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Published:January 2023
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Identification of Salmonella spp. from Colony Picks: ANSR® Salmonella Confirmation Test', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 2013
Final Action 2016
(Applicable to the identification of Salmonella spp. from colony picks from selective/differential agar media: Bismuth sulfite agar, brilliant green sulfa agar, double-modified lysine iron agar, Hektoen enteric agar, tryptic soy agar, xylose lysine deoxycholate agar, and xylose lysine tergitol agar.)
See Tables 2013.14A and B for results of the interlaboratory study supporting acceptance of the method.
Interlaboratory study results for the ANSR Salmonella test: Inclusive isolates
Interlaboratory study results for the ANSR Salmonella test: Exclusive isolates
...
Caution: Use of this test should be restricted to individuals with appropriate laboratory training in microbiology and molecular techniques. Reagents are for laboratory use only. Refer to the Material Safety Data Sheet from Neogen Corp. for more information. Enrichment cultures, used agar plates, and ANSR assay lysates and reaction tubes should be handled and disposed of as potentially infectious material and Biosafety Level 2 measures employed. The preferred method for disposal of contaminated materials, including cultures, pipet tips, tubes, etc., is autoclaving. Items that cannot be autoclaved should be decontaminated by treatment with disinfectant solution. ANSR reaction tubes should not be autoclaved in areas where they may open and possibly contaminate the laboratory environment with amplification products. Alternatively, they may be disposed of in a sealed container with a small amount of 10% household bleach added.
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