
Contents
44.1.15
AOAC Official Method 923.09
Invert Sugar in Sugars and Syrups: Lane-Eynon General Volumetric Method
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Published:January 2023
Cite
Invert Sugar in Sugars and Syrups: Lane-Eynon General Volumetric Method', in Dr. George W Latimer, Jr. (ed.), Official Methods of Analysis of AOAC INTERNATIONAL, 22nd Edition (
Extract
First Action 1923
Final Action
Reagents
Soxhlet modification of Fehling solution.—Prepare by mixing equal volumes of (a) and (b) immediately before use.
...
Standardization
Accurately pipet 10 or 25 mL mixed Soxhlet reagent, or 5 or 12.5 mL each of Soxhlet solutions, A(a) and (b), into 300–400 mL Erlenmeyer. (Amount of Cu taken differs slightly between 2 methods of pipetting, and method used must be consistent in standardization and determination.) Prepare standard solution of pure sugar of such concentration that >15 mL and <50 mL is required to reduce all the Cu. Dispense from buret with offset tip to keep tube out of steam. To determine mg sugar required to completely reduce Cu at different concentrates, consult 930.44 (see Appendix C ) and 930.45 (see Appendix C ). Add sugar solution within 0.5–1.0 mL of total required, heat cold mixture to bp on wire gauze over burner, and maintain moderate boiling 2 min (coarse grains of C or other suitable inert material may be used to prevent bumping). Without removing flame add 1 mL 0.2% aqueous methylene blue solution (or 3–4 drops 1% solution) and complete titration within total boiling time of ca 3 min by small additions (2–3 drops) of sugar solution to decoloration of indicator. (Maintain continuous evolution of steam to prevent reoxidation of Cu or indicator.) After complete reduction of Cu, methylene blue is reduced to colorless compound and solution resumes orange Cu2O color which it had before addition of indicator.
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