
Cover image

On the Cover: In maturing Arabidopsis seeds, embryonic vacuoles are remodelled to become protein storage vacuoles. Shown here, the vacuolar membrane is labelled by the potassium channel TPK1-GFP (green) while storage material accumulating in the lumen is stained with the acidotropic dye Neutral Red (red). Credit: Dr Mistianne Feeney, University of Warwick.
Volume 177, Issue 1, May 2018
ON THE INSIDE
On the Inside
COMMENTARY
The Long and the Short of It: GA 2-oxidaseA9 Regulates Plant Height in Wheat
Improving on the Humble Spud
Photosynthetic Oxygen Production: New Method Brings to Light Forgotten Flux
Turnover of Tonoplast Proteins
UPDATE
Update on Trehalose 6-Phosphate Signaling
The Role of Trehalose 6-Phosphate in Crop Yield and Resilience
T6P can be targeted through genetic and chemical methods for crop yield improvements in different environments through the effect of T6P on carbon allocation and biosynthetic pathways.
BREAKTHROUGH TECHNOLOGIES
Multiplex Fluorescent, Activity-Based Protein Profiling Identifies Active α-Glycosidases and Other Hydrolases in Plants
Activity profiling with biotinylated and fluorescent probes targeting α-glycosidases in plants is applied to saffron crocus and combined with other hydrolase probes.
Chloroplast Ca2+ Fluxes into and across Thylakoids Revealed by Thylakoid-Targeted Aequorin Probes
Aequorin-based probes targeted to the thylakoid lumen and membrane reveal an integrated role for thylakoids in Ca2+ homeostasis and modulation of chloroplast Ca2+ signals.
Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria
Soft x-ray microscopy is a new method developed to determine cellular carbon and nitrogen concentrations in vivo.
Measurement of Gross Photosynthesis, Respiration in the Light, and Mesophyll Conductance Using H2 18O Labeling
H2 18O labeling and IRMS can be used to measure gross photosynthesis, mesophyll conductance, and respiratory uptake in the light in leaflets exposed to 21% and 2% O2.
RESEARCH REPORT
Perception of Sunflecks by the UV-B Photoreceptor UV RESISTANCE LOCUS8
Sunflecks that penetrate through canopy gaps transiently interrupt shade and modify the activity of the UV-B photoreceptor UVR8 to mediate growth and gene expression responses.
Out of Water: The Origin and Early Diversification of Plant R-Genes
Evolutionary analyses suggest that plant R-genes originated in charophytes and plant R-proteins evolved in a modular fashion through frequent gain or loss of protein domains.
Large Crown Root Number Improves Topsoil Foraging and Phosphorus Acquisition
Maize genotypes with more crown roots have more intensive topsoil foraging and superior phosphorus acquisition, growth, and yield in low-phosphorus soil.
Temporal-Specific Interaction of NF-YC and CURLY LEAF during the Floral Transition Regulates Flowering
NF-YC proteins interact with the histone methyltransferase CLF and prevent the trimethylation of H3K27 on chromatin at the FT locus, thereby allowing FT expression and the initiation of flowering.
Articles
BIOCHEMISTRY AND METABOLISM
Reduced Arogenate Dehydratase Expression: Ramifications for Photosynthesis and Metabolism
Modulation of arogenate dehydratase in Arabidopsis causes changes in secondary metabolism, transient overaccumulation of starch, and lower photosynthetic electron transport rates.
Darkened Leaves Use Different Metabolic Strategies for Senescence and Survival
A robust working model delineates the main axes of the divergent metabolic strategies used by Arabidopsis thaliana in response to partial or total darkening treatments.
Exploiting CELLULOSE SYNTHASE (CESA) Class Specificity to Probe Cellulose Microfibril Biosynthesis
Mutagenesis reveals which CESA protein classes have the greatest influence on the regulation of cellulose microfibril biosynthesis in the Arabidopsis secondary cell wall.
Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content
In the wheat Rht18 semidwarf, increased expression of GA2oxA9 metabolizes GA12 precursor to inactive GA110, thereby reducing flux through the GA biosynthetic pathway, resulting in a lower content of bioactive GA and reduced growth.
Betaine Lipid Is Crucial for Adapting to Low Temperature and Phosphate Deficiency in Nannochloropsis
In Nannochloropsis oceanica, betaine lipid is not only important as a surrogate for phospholipids during phosphate deficiency, but is also required for adaptation to low temperature conditions.
Scopoletin 8-Hydroxylase-Mediated Fraxetin Production Is Crucial for Iron Mobilization
In Arabidopsis thaliana, scopoletin 8-hydroxylase mediates the final step in the biosynthesis of fraxetin, a coumarin with a catechol moiety, which is secreted by roots of iron-deficient plants at elevated pH to mobilize iron.
Biotin Attachment Domain-Containing Proteins Irreversibly Inhibit Acetyl CoA Carboxylase
Biotin attachment domain-containing proteins contribute to irreversible inhibition of ACCase under normal growth conditions and under conditions of fatty acid oversupply in Arabidopsis thaliana.
CELL BIOLOGY
The ADAPTOR PROTEIN-3 Complex Mediates Pollen Tube Growth by Coordinating Vacuolar Targeting and Organization
The dynamic organization of vacuoles and the association of tonoplast cargo protein PAT10 are impaired and lead to reduced pollen tube growth in adaptor protein-3 mutants.
ALY RNA-Binding Proteins Are Required for Nucleocytosolic mRNA Transport and Modulate Plant Growth and Development
Arabidopsis expresses four ALY family nuclear RNA-binding proteins that interact with the RNA helicase UAP56, and their depletion causes nuclear mRNA accumulation and developmental defects.
Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles in Arabidopsis thaliana
Preexisting vacuoles are reprogrammed to give rise to protein storage vacuoles.
Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth
RMD, a type II formin, controls pollen tube growth by regulating the direction and distribution of actin filaments arrays in rice (Oryza sativa).
ECOPHYSIOLOGY AND SUSTAINABILITY
The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain
Phosphorus deficiency affects the photosynthetic machinery in barley through a series of sequential events.
GENES, DEVELOPMENT AND EVOLUTION
FERTILIZATION-INDEPENDENT SEED-Polycomb Repressive Complex 2 Plays a Dual Role in Regulating Type I MADS-Box Genes in Early Endosperm Development
A chromatin regulatory complex displays two modes of action in regulating the gene expression of a subclade of a transcription factor family during early endosperm development in Arabidopsis.
RECX Interacts with Mitochondrial RECA to Maintain Mitochondrial Genome Stability
Physcomitrella patens RECX maintains mitochondrial genome stability by interacting with the mitochondrial recombinase RECA1.
The Linker Histone GH1-HMGA1 Is Involved in Telomere Stability and DNA Damage Repair
GH1-HMGA1, an uncharacterised linker histone protein of the High Mobility Group Protein A (HMGA) family, is a telomere-interacting protein and is required for telomere maintenance and DNA double strand break repair.
MEMBRANES, TRANSPORT AND BIOENERGETICS
VIPP1 Involved in Chloroplast Membrane Integrity Has GTPase Activity in Vitro
A chloroplast protein known to be involved in membrane biogenesis and maintenance displays a novel type of GTPase activity requiring oligomerization through its N-terminus.
SIGNALING AND RESPONSE
Polyamines Regulate Strawberry Fruit Ripening by Abscisic Acid, Auxin, and Ethylene
Polyamines play an important role in strawberry fruit ripening by integration of multiple plant hormones, including abscisic acid, auxin, and ethylene.
Magnaporthe oryzae Induces the Expression of a MicroRNA to Suppress the Immune Response in Rice
Magnaporthe oryzae (Guy11) infection induces osa-miR319 expression, which stalls host jasmonic acid signaling and intensifies its pathogenicity.
Heterologous Expression of AtBBX21 Enhances the Rate of Photosynthesis and Alleviates Photoinhibition in Solanum tuberosum
Expression of AtBBX21 in potato causes morphological and physiological changes that improve photosynthetic rates in high-irradiance conditions without negatively affecting water use efficiency.
Ribosomal RNA Biogenesis and Its Response to Chilling Stress in Oryza sativa
Pre-rRNA processing in rice involves two coexisting pathways and responds to chilling stress.
The Potato MAP3K StVIK Is Required for the Phytophthora infestans RXLR Effector Pi17316 to Promote Disease
The RXLR effector Pi17316 from Phytophthora infestans targets a MAP3K, VIK, and supports its activity as a susceptibility factor to promote late blight disease.
Interaction of 2′,3′-cAMP with Rbp47b Plays a Role in Stress Granule Formation
2′,3′-cAMP associates with Arabidopsis Rbp47b and plays a role in stress granule formation.
Systems and Synthetic Biology
Predicted Arabidopsis Interactome Resource and Gene Set Linkage Analysis: A Transcriptomic Analysis Resource
The Predicted Arabidopsis Interactome Resource (PAIR) is a database of high-confidence functional associations between Arabidopsis genes that integrates functional association evidence and enables a network-driven approach for interpreting transcriptomic changes that may functionally impact biological processes.