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ROBERT W. FARMER, DOUGLASS H. BROWN, PATRICK Y. HOWARD, LOUIS F. FABRE, A Radioimmunoassay for Plasma Aldosterone Without Chromatography, The Journal of Clinical Endocrinology & Metabolism, Volume 36, Issue 3, 1 March 1973, Pages 460–465, https://doi.org/10.1210/jcem-36-3-460
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Abstract
A radioimmunoassay for plasma aldosterone has been developed which requires no preliminary chromatography. Purification consists of partitioning between cyclohexane and water, and periodate oxidation followed by a sodium hydroxide wash. The antibody affinity for aldosterone-γ-lactone was approximately 5 orders greater than for other interfering steroids. The specificity of the method was confirmed by the finding that additional purification by Sephadex column chromatography did not lower the observed values. Accuracy was confirmed by recovery studies from pooled plasma containing 70.6 pg/ml (recovery = 0.964 added + 74.9 ± 16.3 pg/ml in the range of 0 to 300 pg/ml added). The coefficient of variation for samples assayed on 5–7 different occasions was 10.6 to 14.1%. This assay is a rapid, efficient and accurate means for measuring plasma aldosterone.