Abstract

The heptafluorobutyrate of tetrahydroaldosterone-γ-lactone was used for the determination of aldosterone in human peripheral plasma by gas chromatography with electron capture detection. The derivative was obtained from plasma as follows: CH2CI2 extraction, cyclohexane/aqueous ethanol partition, HIO4 oxidation to the γ-lactone, thin layer chromatography, enzymatic reduction of the γ-lactone to its tetrahydro derivative, conversion to the heptafluorobutyrate, and thin layer chromatography. The method is relatively simple and sufficiently sensitive to permit assay of aldosterone in normal subjects.

This content is only available as a PDF.
You do not currently have access to this article.