...-to-telomere (T2T) genome for B. blakeana. Comparative chloroplast genome analysis confirmed B. purpurea as the maternal parent. Transcriptome profiling of flower tissues highlighted a closer resemblance of B. blakeana to its maternal parent. Differential gene expression analyses...

...—including the removal of ribosomal RNA, host DNA, and other contaminants—are critical to reducing noise and accurately capturing microbial evidence. We also explore how taxonomic profiling tools, machine learning, and advanced normalization techniques address contamination and low microbial biomass, thereby...

... states, and subsets in an unbiased and precise manner. To address this problem, we designed a 33-color spectral flow cytometry panel for the deep profiling of unprocessed neutrophils in human blood. This panel allows the profiling of neutrophil phenotypes related to activation, immune modulation, granule...

... DNMT3A and TET2 variants, which are associated with clonal hematopoiesis and MPN initiation. One case had a JAK2 p.V617F alteration at a variant allele frequency of 0.9%, below the NGS-reportable range but detectable by PCR, adding another caveat to profiling of MPNs. Discussion...

...Chun Shen Lim; Alexandra K Gibbon; Anh Thu Tran Nguyen; Gabrielle S W Chieng; Chris M Brown Table 1 Performance scores of ribosome profiling analysis tools on eukaryotic data. Datasets Tools Performance scores AUROC AUPRC MCC F1 A. thaliana RIBOSS 0.76 0.51...

... page on our site—for further information please contact [email protected]. Abstract Background Delayed cerebral ischemia (DCI) is a significant complication of aneurysmal subarachnoid hemorrhage (aSAH). This study profiled immune responses after aSAH and evaluated their association...

... female mice. Gene expression profiles were generated from the livers of female mice from the Lang et al. (2020) study treated with the indicated doses of Nafion BP2 (NBP2) by RNA-Seq. Statistically-filtered genes were identified in Partek Flow. A) Principal component analysis. Numbers are in mg/kg/d. B...

... sequencing on the Illumina Miseq platform, we obtained 10 071 318 sequences, which unveiled a diverse and abundant microbial community primarily composed of Proteobacteria, Acidobacteria, and Nitrospirae. Comprehensive taxonomic profiling and gene prediction was carried out using...

... covariate profiles to target specific patient populations and propose methods that progressively integrate weighting and regression modelling. This approach reduces model extrapolation and allows for provider effect modification. We highlight the practical utility of weighting methods that generate stable...

... service planning and implementation. However, few contextual data are available at the village level in rural South and Southeast Asia. This study addressed the gap by profiling representative villages across seven sites in Thailand (n=3), Cambodia, Laos, Myanmar and Bangladesh. Methods Key informant...

... quantitative approaches to overcome the inherent limitations of relative abundances. In this study, we have designed and experimentally validated a set of 12 unique synthetic rRNA operons, which we refer to as rDNA-mimics, to serve as spike-in standards for quantitative profiling of fungal/eukaryotic...

..., ALT, ALP, blood urea nitrogen and creatinine), lipid profiles, antioxidant levels and histopathological studies were investigated. The synthesized NPs showed a spherical shape with an average size of 16–30 nm and exhibited hexagonal structures. Results showed that THQ-loaded ZnONPs resulted...

.../standard-publication-reuse-rights ) biomarkers autoantibody profiling TTV immunoprofiling tocilizumab kidney failure genetic polymorphism Rheumatology key messages Anti-kinesin antibodies and Torque teno virus viral load are promising predictors of relapse in ANCA-associated vasculitis (AAV...

...Renate Kain; André Oszwald NanoString Technologies, Inc. Illumina, Inc. 10.13039/100010905 Rheumatology key messages Spatial profiling in renal or other inflammatory vascular disease allows for the investigation of the transcriptome in defined regions of injury. Protein and mRNA...

... pipelines to improve their performance. Using the Intel VTune profiler, we characterized the bottlenecks of several commonly used MRI preprocessing pipelines from the Advanced Normalization Tools (ANTs), FMRIB Software Library, and FreeSurfer toolboxes. We found few functions contributed to most of the CPU...

.... and Y.Y. were responsible for funding acquisition and resources. Conflict of interest : The authors have no conflicts of interest to disclose. In an attempt to elucidate differences in the regulation of gene expression profiles between the up- and downregulated miRNAs, we conducted pathway and biological...

... 42, phosphorylated tau 181, and total tau but not with Alzheimer’s disease-related neurodegeneration imaging markers, cognitive performance, or clinical diagnosis. PC 2 (explaining 9% of the variance) displayed an inflammatory profile with high contributions of chitinase 3 like 1 (CHI3L1...

... or non-cardiovascular comorbidities. A great heterogeneity of clinical profiles has been described in HFpEF, with several recent studies focused on the identification of different HFpEF phenotypes. In this review, we summarize available evidence on phenotype profiling in HFpEF, describing the different...

.... Additionally, it would enable monitoring of risks associated with potential viral and bacterial infections, and the risks of developing cancers, including post-transplantation lymphoproliferative disorders. Comprehensive monitoring of the immune profile can also be leveraged for potential treatment adjustments...

... gut dysmotility and corticosterone levels following stress exposure, both of which are profoundly disrupted by gut microbiota depletion. Furthermore, metabolomic profiling of serum after microbiota depletion and E. faecalis colonization revealed alterations in metabolites involved in tryptophan...