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Benjamin D Solomon, Chyi-Song Hsieh, Antigen-Specific Development of Mucosal Foxp3+RORγt+ T Cells from Regulatory T Cell Precursors, The Journal of Immunology, Volume 197, Issue 9, November 2016, Pages 3512–3519, https://doi.org/10.4049/jimmunol.1601217
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Abstract
Foxp3+retinoic acid–related orphan receptor (ROR)γt+ T cells have recently been characterized as an immunoregulatory population highly enriched in the colon lamina propria. However, their developmental origin and relationship to RORγt− regulatory T and Th17 cells remain unclear. In this study, we use a fixed TCRβ system to show that the TCR repertoire of the Foxp3+RORγt+ population is mostly distinct compared with other colonic T cell subsets. However, of these TCRs, a fraction is also found in the Th17 subset, suggesting that TCR repertoire overlap may contribute to the reported ability of Foxp3+RORγt+ cells to regulate Th17 immunity. Naive transgenic T cells expressing a Foxp3+RORγt+-restricted TCR first acquire a Foxp3+RORγt− phenotype before coexpressing RORγt, suggesting that Foxp3+RORγt+ cell development can occur via an RORγt− regulatory T cell intermediate.