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Scott M Gordon, Shannon A Carty, Jiyeon S Kim, Tao Zou, Jennifer Smith-Garvin, Eric S Alonzo, Ethan Haimm, Derek B Sant’Angelo, Gary A Koretzky, Steven L Reiner, Martha S Jordan, Requirements for Eomesodermin and Promyelocytic Leukemia Zinc Finger in the Development of Innate-Like CD8+ T Cells, The Journal of Immunology, Volume 186, Issue 8, April 2011, Pages 4573–4578, https://doi.org/10.4049/jimmunol.1100037
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Abstract
Conventional and nonconventional T cell development occur in the thymus. Nonconventional thymocytes that bear characteristics typically associated with innate immune cells are termed innate-like lymphocytes (ILLs). Mice harboring a tyrosine to phenylalanine mutation in the adaptor protein Src homology 2 domain-containing leukocyte protein of 76 kDa at residue 145 (Y145F mice) develop an expanded population of CD8+CD122+CD44+ ILLs, typified by expression of the T-box transcription factor eomesodermin. Y145F mice also have an expanded population of γδ T cells that produce copious amounts of IL-4 via a mechanism that is dependent on the BTB-ZF transcription factor promyelocytic leukemia zinc finger. Using mice with T cell-specific deletion of Eomes, we demonstrate that this transcription factor is required for CD8+ ILL development in Y145F as well as wild-type mice. Moreover, we show that promyelocytic leukemia zinc finger and IL-4 are also required for the generation of this ILL population. Taken together, these data shed light on the cell-intrinsic and cell-extrinsic factors that drive CD8+ ILL differentiation.