-
Views
-
Cite
Cite
Pierre-Joseph Royer, Mohamed Emara, Chaoxing Yang, Abeer Al-Ghouleh, Paddy Tighe, Nick Jones, Herb F Sewell, Farouk Shakib, Luisa Martinez-Pomares, Amir M Ghaemmaghami, The Mannose Receptor Mediates the Uptake of Diverse Native Allergens by Dendritic Cells and Determines Allergen-Induced T Cell Polarization through Modulation of IDO Activity, The Journal of Immunology, Volume 185, Issue 3, August 2010, Pages 1522–1531, https://doi.org/10.4049/jimmunol.1000774
Close - Share Icon Share
Abstract
The mannose receptor (MR) is a C-type lectin expressed by dendritic cells (DCs). We have investigated the ability of MR to recognize glycosylated allergens. Using a gene silencing strategy, we have specifically inhibited the expression of MR on human monocyte-derived DCs. We show that MR mediates internalization of diverse allergens from mite (Der p 1 and Der p 2), dog (Can f 1), cockroach (Bla g 2), and peanut (Ara h 1) through their carbohydrate moieties. All of these allergens bind to the C-type lectin-like carbohydrate recognition domains 4–7 of MR. We have also assessed the contribution of MR to T cell polarization after allergen exposure. We show that silencing MR expression on monocyte-derived DCs reverses the Th2 cell polarization bias, driven by Der p 1 allergen exposure, through upregulation of IDO activity. In conclusion, our work demonstrates a major role for MR in glycoallergen recognition and in the development of Th2 responses.
