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Alix de La Coste, Emmanuelle Six, Nicolas Fazilleau, Laurent Mascarell, Nicolas Legrand, Marie-Pierre Mailhé, Ana Cumano, Yacine Laâbi, Antonio A Freitas, In Vivo and in Absence of a Thymus, the Enforced Expression of the Notch Ligands Delta-1 or Delta-4 Promotes T Cell Development with Specific Unique Effects, The Journal of Immunology, Volume 174, Issue 5, March 2005, Pages 2730–2737, https://doi.org/10.4049/jimmunol.174.5.2730
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Abstract
The role of Notch signaling in T cell commitment during lymphoid development is well established. However, the identity of the ligand that triggers this critical signal in vivo is still unclear. By overexpressing Delta-1 and Delta-4 ligands in the hemopoietic cells of athymic nu/nu host mice, we demonstrate that, in vivo and in the absence of a thymus, Delta-1 or Delta-4 expression is sufficient to promote T cell development from the most immature progenitor stages to complete maturation of both CD8+ and CD4+ αβ T cells. The mature T cells developing in a Delta-1- or Delta-4-enriched environment express a diverse TCR repertoire, are able to proliferate upon in vitro TCR stimulation, but show different profiles of cytokine production after in vitro anti-CD3 stimulation.