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Keisuke Horikawa, Hiroaki Kaku, Hiroshi Nakajima, Helen W Davey, Lothar Henninghausen, Itsuo Iwamoto, Tokutaro Yasue, Ai Kariyone, Kiyoshi Takatsu, Essential Role of Stat5 for IL-5-Dependent IgH Switch Recombination in Mouse B Cells, The Journal of Immunology, Volume 167, Issue 9, November 2001, Pages 5018–5026, https://doi.org/10.4049/jimmunol.167.9.5018
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Abstract
IL-5 stimulation of CD38-activated murine splenic B cells induces μ-γ1 CSR at the DNA level leading to a high level of IgG1 production. Further addition of IL-4 in the system enhances IL-5-dependent μ-γ1 CSR. Although some of the postreceptor signaling events initiated by IL-5 in activated B cells have been characterized, the involvement of Stat in IL-5 signaling has not been thoroughly evaluated. In this study, we examined the activation of Stat5 and activation-induced cytidine deaminase (AID) in CD38-activated murine splenic B cells by IL-5. The role of Stat5a and Stat5b in IL-5-induced μ-γ1 CSR and also IgG1 and IgM production was documented, as IL-5 does not act on CD38-stimulated splenic B cells from Stat5a−/− and Stat5b−/− mice. Expression levels of CD38-induced germline γ1 transcripts and AID in Stat5a−/− and Stat5b−/− B cells upon IL-5 stimulation were comparable to those of wild-type B cells. The impaired μ-γ1 CSR by Stat5b−/− B cells, but not by Stat5a−/− B cells, was rescued in part by IL-4, as the addition of IL-4 to the culture of CD38- and IL-5-stimulated B cells induced μ-γ1 CSR leading to IgG1 production. Analysis of cell division cycle number of wild-type B cells revealed that μ-γ1 CSR was observed after five or six cell divisions. Stat5a−/− and Stat5b−/− B cells showed similar cell division cycles, but they did not undergo μ-γ1 CSR. Our data support the notion that both Stat5a and Stat5b are essential for IL-5-dependent μ-γ1 CSR and Ig secretion; however, their major target may not be AID. Stat5a and Stat5b are not redundant, but rather are at least partially distinctive in their function.
