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Ruey-Chyi Su, Richard G Miller, Stability of Surface H-2Kb, H-2Db, and Peptide-Receptive H-2Kb on Splenocytes, The Journal of Immunology, Volume 167, Issue 9, November 2001, Pages 4869–4877, https://doi.org/10.4049/jimmunol.167.9.4869
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Abstract
We have used flow cytometry to study the stability and peptide-binding capability of MHC class I (MHC-I) on the surface of normal C57BL/6 mouse T lymphoblasts. The MHC-I molecules on each cell are nearly evenly divided into two populations with mean half-life values of ∼1 and 20 h. Our observations suggest that members of the later contain peptide bound with medium to high affinity. Cell surface MHC-I molecules capable of binding exogenous peptide (thus, “peptide-receptive”) belong almost entirely to the less stable population. Before exogenous peptide can bind, MHC-I must undergo a change, probably loss of a very low affinity peptide. For MHC-I-Kb, we found that the maximum rate for binding of exogenous peptide corresponds to a t1/2 value of 12 min. To maintain the 50:50 steady-state distribution of long- vs short-lived MHC-I molecules on the cell surface, ∼20 short-lived molecules must be exported to the cell surface for each long-lived molecule.
