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KEIGO YASUDA, ABBAS E. KITABCHI, Increased Insulin Binding to Erythrocytes in Diabetic Ketoacidosis: Normalization with Insulin Therapy, The Journal of Clinical Endocrinology & Metabolism, Volume 55, Issue 3, 1 September 1982, Pages 408–412, https://doi.org/10.1210/jcem-55-3-408
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Abstract
We studied insulin binding to erythrocytes in eight male type I diabetic patients in diabetic ketoacidosis (DKA) before and 24 h and 5 days after low dose insulin treatment. Mean specific [125I]insulin binding in DKA (mean ± SEM, 13.3 ± 0.6%) was significantly higher than values 24 h and 5 days after treatment (10.7 ± 0.7% and 9.6 ± 0.4%, respectively; P < 0.001). These values after treatment were similar to those of 15 age-matched normal males (10.5 ± 0.4%) and 6 controlled type I diabetic patients (10.1 ± 0.7%). The affinity constant (Ka = 2.26 ± 0.12 109 M−1) was significantly higher in DKA patients than in normal subjects (1.75 ± 0.15 109 M−1) or type I controlled diabetics (1.55 ± 0.15 109 M−1; P < 0.01). Insulin binding correlated inversely with arterial pH (r = −0.80; P < 0.001; n = 8).
These results indicate an increase in specific [125I]insulin binding to erythrocytes in DKA, which is due to elevated receptor affinity (but not receptor numbers) and is reversible with treatment
