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JOHN C. JARRETT, GUSTAVO BALLEJO, JOHN C. M. TSIBRIS, WILLIAM N. SPELLACY, Insulin Binding to Human Ovaries, The Journal of Clinical Endocrinology & Metabolism, Volume 60, Issue 3, 1 March 1985, Pages 460–463, https://doi.org/10.1210/jcem-60-3-460
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Abstract
Human ovarian tissue samples were obtained at the time of laparotomy, and plasma membrane fractions were prepared and used in receptor assays. Incubations of the membrane fraction were performed with [125I]insulin (porcine), and Scatchard analysis of binding showed biphasic curves. The high affinity sites had an average concentration of 57.4 ± 7.9 (±SEM) fmol⁄mg protein and a dissociation constant of 3.5 ± 0.9 nM (n = 9). Neither affinity nor number of binding sites changed significantly during the menstrual cycle. We conclude that there is high affinity binding of [125I]insulin to human ovarian plasma membranes.