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M. L'HERMITE, A. R. MIDGLEY, Radioimmunoassay of Human Follicle-Stimulating Hormone with Antisera to the Ovine Hormone, The Journal of Clinical Endocrinology & Metabolism, Volume 33, Issue 1, 1 July 1971, Pages 68–76, https://doi.org/10.1210/jcem-33-1-68
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Abstract
Since anti-human FSH sera suitable for specific measurement of human FSH (HFSH) in homologous radioimmunoassays (i.e., assays in which the hormone used as antigen and the labeled hormone are from the same species) have been located infrequently, we investigated the possibility of using heterologous systems (i.e., assays in which antisera to a hormone of one species are used in combination with labeled hormone of another species). In the present study, anti-ovine FSH (OFSH) sera from 33 rabbits have been tested for their ability to bind labeled HFSH and to be used subsequently to measure HFSH. Most of the anti-OFSH sera capable of binding appreciable amounts of labeled HFSH could be considered for development of HFSH radioimmunoassays, since the resulting heterologous systems discriminated to a high degree between FSH and HCG. One of these systems has been validated for specific measurement of HFSH. Inhibition curves for sera and pituitary preparations were parallel to each other and to both standards: the 2nd IRP-HMG, of urinary origin, and LER-907, of pituitary origin. The agreement between bioassay and radioimmunoassay estimates of the FSH activity contained in pituitary preparations with widely varying LH to FSH ratios was similar to that obtained with a previously described homologous assay (J Clin Endocr27: 295, 1967). When a pituitary extract was submitted to polyacrylamide gel electrophoresis, the radioimmunoassayable FSH activity was completely separated from LH and from TSH. There was a nearly perfect correlation between FSH estimates of 27 sera determined simultaneously with this heterologous assay and with the aforementioned homologous assay. The likelihood of obtaining a hormone-specific radioimmunoassay may be enhanced with the use of a heterologous system.
