Abstract

An ethanol precipitation of gonadotropins in urine has been combined with a radioimmunoassay for FSH to measure the FSH in the urine of normal males and one patient with the DelCastillo syndrome. Immunologically reactive FSH was recoverable from all urines, except one which was from a hypophysectomized child. Recovery experiments were carried out using the latter urine, to which had been added varying quantities of the Second International Reference Preparation of Human Menopausal Gonadotrophin. Total recoveries were obtained. Serial dilutions of the resuspended protein precipitate also gave proportionate results, verifying the validity of the assay system. It is not known whether the immunological values obtained for FSH are comparable to biological values.

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