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B. J. Meyer, Elmer L. Sevringhaus, Use of Desiccated Blood Pellets in the Biologic Assay of Blood Estrogens, The Journal of Clinical Endocrinology & Metabolism, Volume 3, Issue 10, 1 October 1943, Pages 545–547, https://doi.org/10.1210/jcem-3-10-545
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IN A recent publication, Albrieux (1) described a biological assay of blood estrogen by the intra-vaginal application of desiccated blood pellets. Previously several workers (2–5) had shown that cornification of the vagina of spayed rats can be produced with much smaller doses of estrogens applied directly to the mucosa than can be effected by parenteral administration. Lyons and Temple ton (3) and Yerby (4) were able to produce cornification of the vaginal epithelium of spayed rats by the administration of 1/200 of a rat unit of estradiol benzoate. Albrieux (1) obtained a threshold reaction (induction of cornification although nucleated epithelial cells and leukocytes were still present) in 7 of 12 rats given 0.1 i.u. and in 11 of 12 rats given 0.2 i.u. of an estrone solution made from a sample of the International Standard, administered intravaginally in sesame oil. By administering an acetone-ether extract of whole blood intravaginally in sesame oil, Albrieux was able to demonstrate estrogenic activity equivalent to that of from 5 to 30 i.u. of estrone per 100 cc. of blood.