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IRA D. GOLDFINE, S. M. AMIR, ANN W. PETERSEN, SIDNEY H. INGBAR, Preparation of Biologically Active 125I-TSH, Endocrinology, Volume 95, Issue 5, 1 November 1974, Pages 1228–1233, https://doi.org/10.1210/endo-95-5-1228
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The present study was performed to determine whether iodinated bovine TSH of high specific radioactivity could be prepared by a method that would permit retention of its biological activity. Using stoichiometric concentrations of TSH, Nal, and chloramine T, the hormone was iodinated or radioiodinated to the extent of 1iodine atom per molecule. Iodinated TSH was then purified from the reaction mixture by sequential chromatography on Sephadex G-25 and G-100. 127I-TSH had full potency both in the TSH radioimmunoassay and in the McKenzie bioassay. I25I-TSH also had full potency in the bioassay and at least 70% of the label bound to purified bovine thyroid membranes. These studies indicate that iodinated TSH retains biological activity when prepared by the method employed, and that radioiodinated TSH prepared in this manner is a suitable tool for studying the binding of TSH to its tissue receptors. (Endocrinology95: 1228, 1974)
