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FUSUN N. ZEYTIN, SCOTT F. RUSK, VINCENT RAYMOND, ARNOLD J. MANDELL, Fibroblast Growth Factor Stabilizes Ribonucleic Acid and Regulates Differentiated Functions in a Multipeptide-Secreting Neuroendocrine Cell Line, Endocrinology, Volume 122, Issue 3, 1 March 1988, Pages 1121–1128, https://doi.org/10.1210/endo-122-3-1121
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A clonal cell line (44-2C) which synthesizes and secretes somatostatin, neurotensin, calcitonin (CT), and CT gene-related peptide and transiently expresses c-fos was used to characterize the mechanism of action of basic fibroblast growth factor (bFGF). bFGF had two modes of action: 1) short term incubation of 44-2C cells with bFGF increased the cellular content of neurotensin, somatostatin, and CT; and 2) bFGF enhanced the response of the cells to rat hypothalamic GRFmediated cAMP efflux. The long term action of bFGF was manifested by the permissive effect of the molecule. bFGF had a sustained effect on RNA synthesis, and pretreatment with bFGF for 24 h altered the time course of response of the cells to rat GRF. In this cell line the cellular action of bFGF was not mediated via protein kinase-C action. bFGF was not mitogenic in 44-2C cells. bFGF stimulated uridine incorporation without affecting thymidine incorporation. Results obtained with actinomycin- D and a-amanitin suggest that the above effects of bFGF can be correlated with increased RNA stability produced by bFGF. (Endocrinology122: 1121–1128, 1988)