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JOSEP G. LLAURADO, Some Effects of Aldosterone on Sodium Transport Rate Constants in Isolated Arterial Wall: Studies with Computer Simulation and Analysis, Endocrinology, Volume 87, Issue 3, 1 September 1970, Pages 517–526, https://doi.org/10.1210/endo-87-3-517
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Abstract
Transport rate constants (k) describing the kinetics of Na exchanges in isolated dog's carotid wall were determined with the aid of 22Na as a tracer and digital computer simulation without recourse to ancillary chemical measurements of extracellular space. Results show that aldosterone (0.85ì10-6M) added to the incubating medium causes the following statistically significant changes on the arterial wall: (i) a 35% diminution of the over-all Na outflow rate constant kOi; (ii) a 3-fold diminution of the Na transport rate constant from extra to intracellular space k-2i; (Hi) a doubling of the ratio of extra to intracellular Na. These results indicate that aldosterone induces an accumulation or translocation of Na into the extracellular space. The present findings are related to those reported previously by other workers in various tissues. Results further suggest that aldosterone acts on passive Na transport impeding the entry of this cation into cells. The main significance of the approach followed in this work is that the values found for the intercompartmental transport rate constants are not subject to fluctuations intrinsic to auxiliary indicators of extracellular space. (Endocrinology87: 517, 1970)