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KEVIN MORGAN, STEVEN M. FOORD, GILLIAN SPURLOCK, BAMBOS M. CHARALAMBOUS, CARLOS DIEGUEZ, MAURICE F. SCANLON, M. AFZAL MIR, RELEASE OF AN ACTIVE SODIUM TRANSPORT INHIBITOR (ASTI) FROM RAT HYPOTHALAMIC CELLS IN CULTURE, Endocrinology, Volume 115, Issue 4, 1 October 1984, Pages 1642–1644, https://doi.org/10.1210/endo-115-4-1642
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To investigate the hypothesis whether the hypothalamus releases an active (ouabain-sensitive) sodium transport inhibitor, we cultured hypothalamic and cortical cells from day 17 fetal rats. Culture media from hypothalamic cells reduced the total erythrocyte sodium efflux rate constant from 0.487 ± (se) 0.014 to 0.408 ± 0.013 (P < 0.001), and the ouabain-sensitive rate constant from 0.305 ± 0.015 to 0.240 ± 0.016 (P < 0.01). HypothaTamic media also showed a dose-dependent displacement of [3H]-ouabain-binding to erythrocyte membranes. Neither cortical nor conditioned media (incubated without cells) had any effect. Various well-characterized hormones of hypothalamic origin failed to inhibit sodium efflux rate constant. These studies demonstrate that fetal rat hypothalamic cells contain and release a factor which inhibits sodium transport in human erythrocytes,