Abstract

ALDEHYDES in the tissues were demonstrated by their reaction with Fuchsin Sulfurous Acid (FSA) by Feulgen and Voit (1924). They were found to consist mainly of stearal and palmital linked in a cyclic acetal bond to glycerol-phosphoryl-cholamine with the following structure (Feulgen and Bersin, 1939): RHC-OCH2-CHO-CH2-

OPO(OH)OCH2CH2-NH2. They are present in various tissues in most animals. Oster and Mulinos (1944) showed that tissue aldehydes exhibit a characteristic distribution pattern in kidneys of rats, guinea pigs, rabbits, cats and dogs, when stained with FSA.

In the cortex all glomeruli stain lightly, and only isolated tubules stain deeply, those remaining being almost unstained. The deepest and most uniform staining is seen in the intercortico-medullary zone (ICM zone). The Henle's loop region of the medulla stains less deeply, and groups of unstained collecting tubules give a spoke-like appearance to the loop bundles. The pelvic portion of the medulla is almost unstained.

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