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Makio Ogawa, Humoral regulation of early hemopoiesis studied in culture, The International Journal Of Cell Cloning, Volume 9, Issue S1, 1991, Pages 72–78, https://doi.org/10.1002/stem.5530090710
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Abstract
Hemopoietic proliferation appears to be controlled by a cascade of growth factors, each directed at a specific stage of development. In this model, proliferation of multipotential progenitors is regulated by such factors as interleukin (IL) 3, IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). Studies in our laboratory, however, indicated that IL-3 does not trigger cell cycling of dormant stem cells but rather supports proliferation of multipotential progenitors only after they exit from the dormant state. Subsequently, we identified several growth factors that appear to shorten the period of dormancy (G0) of early hemopoietic progenitors. These include IL-6, granulocyte (G) CSF, IL-11 and the ligand for c-kit. IL-11 was originally cloned from a primate stromal cell line, PU-34, and possesses multiple biological functions that are similar to IL-6. It works synergistically with IL-3 or IL-4 in support of proliferation of multipotential blast cell colonies. Stem cell factor (SCF) that was identified and cloned from buffalo rat liver cell line proved to be the ligand for c-kit. SCF enhances IL-3-dependent but not IL-4-dependent proliferation of early progenitors. SCF was also found to collaborate with IL-6 or G-CSF in support of proliferation of multipotential progenitors. These observations indicate the importance of stromal cells in regulating the very early process of stem cell proliferation.
