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Hailan Zhang, Johnathan L. Nieves, Stuart T. Fraser, Joan Isern, Panagiotis Douvaras, Dmitri Papatsenko, Sunita L. D'Souza, Ihor R. Lemischka, Michael A. Dyer, Margaret H. Baron, Expression of Podocalyxin Separates the Hematopoietic and Vascular Potentials of Mouse Embryonic Stem Cell-Derived Mesoderm, Stem Cells, Volume 32, Issue 1, January 2014, Pages 191–203, https://doi.org/10.1002/stem.1536
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Abstract
In the mouse embryo and differentiating embryonic stem cells, the hematopoietic, endothelial, and cardiomyocyte lineages are derived from Flk1+ mesodermal progenitors. Here, we report that surface expression of Podocalyxin (Podxl), a member of the CD34 family of sialomucins, can be used to subdivide the Flk1+ cells in differentiating embryoid bodies at day 4.75 into populations that develop into distinct mesodermal lineages. Definitive hematopoietic potential was restricted to the Flk1+Podxl+ population, while the Flk1-negative Podxl+ population displayed only primitive erythroid potential. The Flk1+Podxl-negative population contained endothelial cells and cardiomyocyte potential. Podxl expression distinguishes Flk1+ mesoderm populations in mouse embryos at days 7.5, 8.5, and 9.5 and is a marker of progenitor stage primitive erythroblasts. These findings identify Podxl as a useful tool for separating distinct mesodermal lineages. Stem Cells 2014;32:191–203