Abstract

In the latent period of the induction of murine erythroleukemia (MEL) cell differentiation, c-myc mRNA levels show a drastic change. The elevated expression of a transfected c-myc gene inhibits the commitment and terminal differentiation of MEL cells, c-myc regulates commitment and differentiation by means of its different functional domains: almost all regions are required for inhibition of commitment, whereas domains II and IV are required for inhibition of terminal differentiation. c-myc may regulate commitment and differentiation by interacting with proteins through different domains. In addition to differential regulation of the latent-period genes, overexpression of c-myc represses the expression of glycophorin, globin and δ-aminolevulinic acid synthase-erythroid (ALAS-E) genes, but it has no effect on the expression of GATA-1 and erythropoietin receptor (EpoR) genes. A possible mechanism for the coordinated regulation of globin and heme synthesis is discussed.

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