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Peter van Wezenbeek, Pieter Vos, Jacques Van Boom, Albert van Kammen, Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA, Nucleic Acids Research, Volume 10, Issue 24, 20 December 1982, Pages 7947–7957, https://doi.org/10.1093/nar/10.24.7947
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Abstract
Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon Cloning in bacteriophage. M13mp9, two recombinant phages were selected to construct a p8R322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously establishment sequence of another PSTV strain, three of which were base exchanges and one a deletion.
Author notes
* Present address: Laboratory of Biochemistry, University of Nijmegen, 6525 EZ Nijmegen, The Netherlands
+ Gorlaeus Laboratories, State University of Leiden, P.O. BoX 9502, 2300 RA Leiden The Netherlands
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