Sequences of four tRNA genes from Caenorhabditis elegans and the espression of C.elegans tRNA ^Leu (anticodon IAG) in Xenopus oocytes Open Access

Four tRNA genes have been identified in cloned segments of Caenorhabditis elegans DNA by tRNA hybridization and expression after injection into Xenopus laevis oocyte nuclei. From DNA sequencing these are (with DNA anticodon sequences) tRNA ^Asp (CTC), tRNA ^Leu (AAC), tRNA ^Lys (CTT) and tRNA ^Pro (TGG). Their flanking DNA sequences are compared. Two identical tRNA ^Lys (CTT) genes from different regions of the genome have quite unrelated 5‘ flanking sequences the tRNA synthesized in Xenopus oocytes after injection of the tRNA ^Leu cloned DNA has the modified anticodon JAG. The tRNA ^Leu gene precursor transcript from injected oocytes has short 5‘ and 3‘ additional sequences and Jacks certain of those modified bases found in the processed tRNA.