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Renato Pérez-Rosés, Ester Risco, Roser Vila, Pedro Peñalver, Salvador Cañigueral, Antioxidant activity of Tween-20 and Tween-80 evaluated through different in-vitro tests, Journal of Pharmacy and Pharmacology, Volume 67, Issue 5, May 2015, Pages 666–672, https://doi.org/10.1111/jphp.12369
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Abstract
This study aimed to evaluate the possible antioxidant activity of Tween-20 and Tween-80, two amphipathic nonionic surfactants commonly used as solubilizers and stabilizers, whose pharmacological effects have been ignored to a large degree.
Antioxidant activity was investigated in vitro measuring the scavenging activity on the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH●), the production of reactive oxygen species (ROS) in activated human neutrophils using flow cytometry and the myeloperoxidase (MPO) inhibitory activity.
Tween-20 and Tween-80 did not show scavenging activity on DPPH●, while produced a decrease of the ROS production in human neutrophils, being Tween-20 more active than Tween-80. Moreover, Tween-80 and Tween-20 were found to significantly stimulate MPO enzymatic activity.
Our findings raise concerns with regard to the indiscriminate use of Tween-20 and Tween-80 in clinical and laboratory testing, since they could influence the results that are assigned to the tested substance.