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Pijus K Barman, Jingbo Pang, Norifumi Urao, Timothy J Koh, Skin Wounding–Induced Monocyte Expansion in Mice Is Not Abrogated by IL-1 Receptor 1 Deficiency, The Journal of Immunology, Volume 202, Issue 9, May 2019, Pages 2720–2727, https://doi.org/10.4049/jimmunol.1801481
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Abstract
The aim of this study was to determine whether skin wounding induces monocyte (Mo) expansion in bone marrow and whether IL-1R1 signaling regulates this process. Our data show that skin wounding increases myeloid lineage–committed multipotent progenitors (MPP3 subset) and Mo in bone marrow, but this expansion is not impaired in Il1r1−/− mice. We also demonstrate that M-CSF–induced differentiation of myeloid progenitors into Mo is not impaired by the loss of IL-1R1 ex vivo, indicating that IL-R1 deficiency does not abrogate myeloid progenitor differentiation potential. In addition, we observed modestly delayed wound closure in Il1r1−/− mice associated with higher frequency of Ly6Clo Mo in the circulation at baseline and in wounds early after injury. Thus, in contrast to other models of inflammation that involve IL-1R1–dependent monopoiesis, our results demonstrate that skin wounding induces Mo progenitor and Mo expansion independently of IL-1R1 signaling.