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Andrea Bottaro, Igor Kuzin, Stephen Kates, Yawen Wu, Homaira Rahimi, Wojciech Wojciechowski, Steven Bernstein, Richard Burack, Edward Schwarz, Accumulation of CD23+ CD21-high Bin-like cells in human reactive and rheumatoid arthritis lymph nodes, The Journal of Immunology, Volume 196, Issue 1_Supplement, May 2016, Page 124.10, https://doi.org/10.4049/jimmunol.196.Supp.124.10
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Abstract
We have previously described a unique population of CD23+ CD21-high B cells in inflamed nodes (Bin) which accumulate in lymph nodes (LN) draining inflamed joints of TNF-transgenic (TNF-tg) mice, and contribute to arthritis flare by distorting node architecture and hampering lymphatic flow. The existence of Bin-like cells in human inflamed LN is yet unknown. Here we report the characterization of resident B cell populations in fresh popliteal lymph nodes (PLN) harvested from discarded tissues from rheumatoid arthritis (RA) patients and patients with other severe lower limb diseases (non-RA), undergoing total knee arthroplasty or lower limb amputation. We also analyzed banked, cryopreserved reactive and normal human LN single cell suspension samples. Bin-phenotype cells were shown to be significantly increased in reactive LN, and strikingly elevated (>30% of total) in RA samples. Histopathology and immunofluorescence analyses were consistent with Bin expansion and histological alterations in RA vs. non-RA PLN. This is the first description of Bin-like cells in human inflamed LN. Consistent with published mouse data, this population appears to be associated with inflammatory arthritis and distortion of LN architecture. Further analysis of this population will be important to assess its role in RA pathogenesis and arthritic flare.
