Abstract

C5L2 is a recently identified receptor for C5a and C3a/C3adesArg (ASP). C5a/C5adesArg binds C5L2 with high affinity but no functional activation. By contrast, C5L2 is a functional receptor for ASP and in adipocytes plays a role in lipid metabolism. In this study, we used adipose tissue microarray analysis to explore the overlap between metabolism and immunology. Subcutaneous (SC AT) and omental (OM AT) adipose tissue were collected from 14 subjects (normal + obese) and divided into two groups based on plasma ASP level (assayed by ELISA): high ASP group (&#8593;ASP) (ASP>40nM) and low ASP group (&#8595;ASP ) (ASP<40 nM).

C5L2 (p=0.06), C5aR (p=0.03), IL-6 (p=0.01), and IL-6R (p=0.03) expression were increased significantly in &#8593; ASP vs. &#8595;ASP in OM AT, with no change in SC AT. Plasma ASP correlated with lipid metabolic factors GPAT-1, FABP1, FABP5, FABP7 and adipophilin, and inflammatory factors CRP, C5aR and IL-6R in OM AT. ASP was mainly correlated with lipid metabolic factors, such as LPL, CD36, FABP3, and FABP6 in SC AT.

ASP-C5L2 may play a role in mediating metabolic- immune interactions in adipose tissue.

Funding: CIHR

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