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Guangwen Ren, Gobardhan Das, Liying Zhang, Arthur Roberts, Yufang Shi, Mechanisms Study on Tolerogenic Dendritic Cells Induced by Apoptotic Cells (B161), The Journal of Immunology, Volume 178, Issue 1_Supplement, April 2007, Page LB34, https://doi.org/10.4049/jimmunol.178.Supp.B161
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Abstract
Apoptosis plays a pivotal function in maintaining the homeostasis of the immune system. Our previous experiments have demonstrated that i.v. administration of apoptotic cells of donor origin significantly prolonged the survival of allograft. However, the mechanism was unknown. In this report, we found that dendritic cells (DCs) play an important role in apoptotic cells-mediated immunosuppression. DCs fed with apoptotic lymphocytes (DCaps) down-regulated expression of MHC class II and costimulatory molecules CD80, CD86, as well as producing less IL-6, IL-12 and TNFalpha, but more IL-10. When DCaps were presented with antigen and then injected into the footpad of syngenic mice, they induced a much weaker immune response in draining lymph nodes compared to normal DCs. However, DCaps exhibited much less suppressive effect in ex vivo stimulation for CD4+ T cells in the presence of anti-CD3 when compared with the in vivo model. Surprisingly, the mixture of both normal DCs and DCaps could strikingly suppress T cells proliferation ex vivo. Further studies on the mechanisms of the helper role of normal DCs toward DCaps to get the immunosuppressive effect will strengthen the understanding of apoptotic cells-mediated immune tolerance.