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Gillian A Lang, Mark L Lang, Protein Kinase Bα Is Required for Vesicle Trafficking and Class II Presentation of IgA Fc Receptor (CD89)-Targeted Antigen, The Journal of Immunology, Volume 176, Issue 7, April 2006, Pages 3987–3994, https://doi.org/10.4049/jimmunol.176.7.3987
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Abstract
Ag presentation stimulates Ag-specific adaptive immune responses. FcαR (CD89)-mediated capture of IgA-bound exogenous Ag leads to efficient MHC class II Ag presentation by APCs. CD89 signaling is required for trafficking of internalized Ag to specialized multivesicular bodies known as MHC class II compartments (MIIC) and subsequent class II presentation. In the present study, we tested the hypothesis that the vesicle trafficking regulator protein kinase Bα (PKBα) is required for CD89-mediated trafficking to MIIC and Ag presentation. We observed by two independent methods (chemical inhibitors and specific RNA interference) that PKBα was required for CD89 trafficking to MIIC and class II Ag presentation. Expression of constitutively active PKBα in APCs expressing a mutant CD89 accessory signaling molecule (deficient in CD89/Ag trafficking, processing, and presentation) induced trafficking of CD89 to lamp1-containing late endocytic vesicles, but not class II-containing vesicles (MIIC), or class II Ag presentation. These studies show for the first time that PKBα is required for receptor-mediated Ag presentation and suggest the mechanism of action includes regulation of vesicle trafficking.