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Patricia de Nadaï, Anne-Sophie Charbonnier, Cécile Chenivesse, Stéphanie Sénéchal, Clément Fournier, Jules Gilet, Han Vorng, Ying Chang, Philippe Gosset, Benoı̂t Wallaert, André-Bernard Tonnel, Philippe Lassalle, Anne Tsicopoulos, Involvement of CCL18 in Allergic Asthma, The Journal of Immunology, Volume 176, Issue 10, May 2006, Pages 6286–6293, https://doi.org/10.4049/jimmunol.176.10.6286
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Abstract
Allergic asthma is associated with a pulmonary recruitment of Th type 2 cells, basophils, and eosinophils, mainly linked to chemokine production. CCL18 is a chemokine preferentially expressed in the lung, secreted by APCs, induced by Th2-type cytokines, and only present in humans. Therefore, CCL18 may be involved in allergic asthma. PBMC from asthmatics allergic to house dust mite cultured in the presence of Dermatophagoides pteronyssinus 1 (Der p 1) allergen secreted CCL18, 48 and 72 h after stimulation, whereas those from healthy donors did not. Part of CCL18 was directly derived from Der p 1-stimulated plasmacytoid dendritic cells, whereas the other part was linked to monocyte activation by IL-4 and IL-13 produced by Der p 1-stimulated T cells. In bronchoalveolar lavages from untreated asthmatic allergic patients, CCL18 was highly increased compared with controls. Functionally, CCL18 preferentially attracted in vitro-polarized Th2 cells and basophils, but not eosinophils and Th1 cells, and induced basophil histamine and intracellular calcium release. These data show a new function for CCL18, i.e., the recruitment of Th2 cells and basophils, and suggest that CCL18 may play a predominant role in allergic asthma.