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A. Fournier, M. E. Cohen Solal, R. Oprisiu, H. Mazouz, P. Morinire, G. Choukroun, R. Bouillon, Optimal Range of Plasma Concentration of True 1–84 Parathyroid Hormone in Patients on Maintenance Dialysis, The Journal of Clinical Endocrinology & Metabolism, Volume 86, Issue 4, 1 April 2001, Pages 1840–1846, https://doi.org/10.1210/jcem.86.4.7436-8
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To the editor:
We have read with interest the rapid communication of John et al. (1) showing that the new Scandibodies laboratory immunoradiometric assay (IRMA) for measurement of intact PTH detects full-length human PTH but not amino-terminally truncated fragments, in contrast to most commercially available IRMAs for so-called intact PTH, which measure also 7–84 PTH as shown by Lepage et al. (2).
In contrast to the Nichols Institute Allegro IRMA for intact PTH (N-IRMA), our PTH IRMA (B-IRMA) (Ref. 3 ; later commercialized by Biosource for the last 10 yr) had the particularity to have a much lower normal range of 3–40 pg/mL vs. 10–65 pg/mL for N-IRMA. The reason for this discrepancy was not clear until 1992 when we compared the recovery of 7–84 PTH fragments at a wide concentration range in our assay and in that of Nichols Institute. The recovery of fragment (7–84) was 100% with N-IRMA and less than 1% in B-IRMA. Therefore, the nondetection of 7–84 was a satisfactory explanation for the lower normal range.
