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ERIKO NISHINO, NOBORU MATSUZAKI, KAZUO MASUHIRO, TAKASHI KAMEDA, TAKESHI TANIGUCHI, TETSU TAKAGI, FUMITAKA SAJI, OSAMU TANIZAWA, Trophoblast-Derived Interleukin-6 (IL-6) Regulates Human Chorionic Gonadotropin Release through IL-6 Receptor on Human Trophoblasts, The Journal of Clinical Endocrinology & Metabolism, Volume 71, Issue 2, 1 August 1990, Pages 436–441, https://doi.org/10.1210/jcem-71-2-436
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Abstract
We examined the capacity of trophoblast-derived interleukin-6 (IL-6) to stimulate secretion of placental hormones, including hCG. IL-6 stimulated hCG secretion by trophoblasts to a level similar to that stimulated by a GnRH analog. The analog, however, released hCG by an IL-6-independent mechanism because PM-1, a monoclonal antibody specific for IL-6 receptors (R), failed to block GnRH-mediated responses, but completely blocked IL-6-mediated hCG secretion, suggesting the existence of two distinct regulatory pathways for hCG release. Immunohistochemical analysis with another IL-6-R-specific antibody, MT-18, showed that IL-6-R was located only on the trophoblast layer of the placenta. Our data revealed the existence of a local regulatory network by which trophoblastderived IL-6 interacts with IL-6-R on the trophoblasts, resulting in hCG release. Thus, two different regulatory networks, an IL-6 and IL-6-R system and a GnRH and GnRH-R system, regulate hCG release by human trophoblasts independently.