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NOBUAKI KUZUYA, HIDEMASA UCHIMURA, HITOSHI IKEDA, SHOO CHENG CHIU, NOBORU HAMADA, KUNIHIKO ITO, SHIGENOBU NAGATAKI, Adenosine 3′,5′-Monophosphate Concentrations and Responsiveness to Thyrotropin and Thyroid-Stimulating Immunoglobulins in Normal and Graves' Thyroids, The Journal of Clinical Endocrinology & Metabolism, Volume 51, Issue 1, 1 July 1980, Pages 59–63, https://doi.org/10.1210/jcem-51-1-59
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Abstract
cAMP concentrations and cAMP responses to TSH and serum thyroid stimulators were determined in normal thyroids and thyroids of patients with Graves' disease. cAMP concentrations in tissues obtained from patients with Graves' disease treated with thionamide drugs and iodide were significantly greater than those in normal tissues obtained from euthyroid patients with solitary cold nodules (normal: 0.27 ± 0.02 pmol/mg tissue, n = 40; Graves': 0.50 ± 0.06, n = 17, P < 0.001). Responses of cAMP to TSH were determined using thyroid slices preincubated with theophylline for 30 min and incubated with or without TSH for 10 min; responses were expressed as the ratio of cAMP concentrations in slices with and without TSH. Responses to TSH were significantly decreased in thyroids obtained from thionamide-treated patients with Graves' disease regardless of preoperative iodide therapy (normal: 593 ± 60%, n = 26; Graves' with iodide therapy: 267 ± 49%, n = 13, P < 0.001; Graves' without iodide therapy: 347 ± 84%, n = 17, P < 0.05). Responses of cAMP to serum thyroid stimulators were determined using thyroid slices incubated for 2 h with 10 mg immunoglobulin G (IgG) obtained from normal subjects or untreated patients with Graves' disease and were expressed as the ratio of cAMP concentrations in slices incubated with Graves' IgG and normal IgG. Responses of cAMP to serum thyroid stimulators were significantly lower in thyroids obtained from thionamidetreated patients with Graves' disease than in normal thyroids (normal: 779 ± 97%, n = 5; Graves': 132 ± 14%, n = 5, P < 0.001). There results indicate that in the thyroid glands of patients with Graves' disease rendered eumetabolic with thionamide therapy, there is still continuing stimulation of the tissue, at least as evidenced by high baseline cAMP values, and lower responses to TSH or Graves' IgG than those seen in normal thyroid glands.
