Abstract

A radioimmunoassay is described for the measurement of 17α-hydroxyprogesterone in human plasma. We have employed the principle of “immunologic purification.” 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether. The 17-OHP is subsequently freed from antibody by acid hydrolysis and this extract is assayed by radioimmunoassay. (J Clin Endocrinol Metab40: 863, 1975)

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