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MIRIAM HAMI, ARIEL ROSLER, DAVID RABINOWITZ, A Nonchromatographic Radioimmunoassay for 17α-Hydroxyprogesterone, The Journal of Clinical Endocrinology & Metabolism, Volume 40, Issue 5, 1 May 1975, Pages 863–867, https://doi.org/10.1210/jcem-40-5-863
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Abstract
A radioimmunoassay is described for the measurement of 17α-hydroxyprogesterone in human plasma. We have employed the principle of “immunologic purification.” 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether. The 17-OHP is subsequently freed from antibody by acid hydrolysis and this extract is assayed by radioimmunoassay. (J Clin Endocrinol Metab40: 863, 1975)
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Copyright © 1975 by The Endocrine Society
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