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R. HOBKIRK, MONA NILSEN, D. G. WILLIAMSON, D. S. LAYNE, Metabolism of Intravenously Administered 17β-Estradiol-6,7-3H-3-glucoside-14C in Normal Women, The Journal of Clinical Endocrinology & Metabolism, Volume 32, Issue 4, 1 April 1971, Pages 476–480, https://doi.org/10.1210/jcem-32-4-476
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Abstract
17β-Estradiol-6,7-3H-3-glucoside-14C (uniformly labeled) was prepared and injected intravenously into 2 normal young women. Within about 2 hr 20% of the dose was excreted in the urine of each subject in the form of estrogen glucoside with a substantially unchanged 3H/14C ratio. Approximately 11% of the latter was identified as estrone-3-glucoside, the remainder being the compound injected. 3H-labeled estrogen glucosiduronates and, to a smaller extent, 3H-estrogen sulfates, were also excreted during the first 2 hr and these metabolites continued to appear in the urine, unaccompanied by 14C, up to and including the fourth day after injection. Recovery of 3H in the urine (including that associated with the urinary glucosides) was 60 and 81% in the 2 subjects. The steroid pattern identified in the urinary 3H-glucosiduronate fraction indicated that the major part of the injected 17β-estradiol-3-glucoside had been hydrolyzed, and that the 17β-estradiol released was metabolized in a manner similar to that of intravenously injected 17β-estradiol.