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Wilbur A. Robbie, Robert B. Gibson, Rapid Clinical Determination of Urinary 17-Ketosteroids, The Journal of Clinical Endocrinology & Metabolism, Volume 3, Issue 4, 1 April 1943, Pages 200–205, https://doi.org/10.1210/jcem-3-4-200
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Abstract
DETERMINATION of the 17-ketosteroid content of urine specimens has been a lengthy procedure because of the time required for extraction and the detailed process involved in the purification and measurement of the extract. Since the normal variation between individuals is great and since there is considerable change from the normal in clinical conditions in which the test may be used as a diagnostic aid, it seems justifiable to place more emphasis on convenience and speed than on matters of detail that might add slightly to accuracy. The following article describes more fully the method previously outlined by the authors (1). An effort has been made to reduce to a minimum the time required for the determination without a corresponding decrease in accuracy. The resulting method is simple and rapid and yet compares favorably with other published techniques in completeness of recovery and reproducibility of results.
The method is used in determining the 17-ketosteroid content of a urine sample.