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KENNETH J. RYAN, ZOLTAN PETRO, JURGEN KAISER, Steroid Formation by Isolated and Recombined Ovarian Granulosa and Thecal Cells, The Journal of Clinical Endocrinology & Metabolism, Volume 28, Issue 3, 1 March 1968, Pages 355–358, https://doi.org/10.1210/jcem-28-3-355
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Abstract
Granulosa and thecal cells were isolated from 2 human ovarian follicles and the separated and recombined cells were incubated in vitro with acetate-1-14C. Radioactively labeled cholesterol, pregnenolone, progesterone, 17-hydroxyprogesterone, androstenedione and estrone were isolated in radiochemically pure form from each of the experimental situations utilizing either granulosa, theca or a mixture of the 2 cell types. In addition, labeled dehydroepiandrosterone was isolated only from the incubation with granulosa cells, while labeled estradiol-17β was isolated only from the thecal cell and recombined studies.