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JOHN A. RESKO, KRISTEN B. EIK-NES, Diurnal Testosterone Levels in Peripheral Plasma of Human Male Subjects, The Journal of Clinical Endocrinology & Metabolism, Volume 26, Issue 5, 1 May 1966, Pages 573–576, https://doi.org/10.1210/jcem-26-5-573
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A diurnal variation of testosterone in the peripheral plasma of adult male subjects has been observed. Significantly greater quantities of the hormone (p<0.01) were found at time 0800 when compared to the levels in the afternoon or in the evening. Also, the plasma concentration of testosterone was higher on 2 consecutive mornings compared to the intervening evening. The magnitude of this diurnal variation was not so great as that reported for plasma Porter-Silber chromogens or for conjugated dehydroepiandrosterone.